|
週次 |
日期 |
單元主題 |
|
第1週 |
2/26 |
Course introduction
Protein structure (Chap 2): introduce amino acids, protein secondary structure
Build up secondary structures using amino acid models (Practice, homework)
授課老師：陳佩燁 |
|
第2週 |
3/05 |
(Bring computer) Use molecular modeling software to display your protein (SwissPDBviewer) (Practice, homework)
Topology and motif
Protein folding theory, protein structural stability, protein folding kinetics
授課老師：陳佩燁 |
|
第3週 |
3/5 + 3/19 |
Chaperone
Protein misfolding, amyloid, misfolding-related diseases
授課老師：陳佩燁 |
|
第4週 |
3/19 |
Understanding cell biology from structural biology aspect (Chap 11)
Importance of visualization
Seeing molecular mechanism in cell signaling
How protein kinases work
授課老師：何孟樵 |
|
第5週 |
3/26 |
Protein expression
Basic concept of protein biogenesis
Practical consideration of recombinant protein production
What can you do if you doesn’t get your protein
授課老師：何孟樵 |
|
第6週 |
4/2 |
兒童節遇例假日補假 |
|
第7週 |
4/9 |
Protein crystallography
The importance of resolution
How to visualize/evaluate the result
General concept of protein crystallography
What protein crystallography can and cannot do
授課老師：何孟樵 |
|
第8週 |
4/16 |
Introduce membrane protein (Chap 10)
Preparation of membrane proteins for structural studies
授課老師：徐尚德 |
|
第9週 |
4/23 |
期中考 |
|
第10週 |
4/30 |
Use NMR to solve protein structure Part I
Sample requirement, sample preparation, limitation of the technique, isotope-labeling)
(Practice) How to assign a 2D-NMR spectrum
授課老師：徐尚德 |
|
第11週 |
5/7 |
Use NMR to solve protein structure Part II
How to use NMR to examine protein-protein or protein-ligand interaction
Hydrogen-deuterium exchange NMR and mass
授課老師：徐尚德 |
|
第12週 |
5/14 |
Biological NMR spectroscopy and hands-on tutorial of HDX analysis by Sparky
授課老師：徐尚德 |
|
第13週 |
5/21 |
Bioinformatics, database and analysis.Protein structure prediction and analysisIntroduction of intrinsically disordered protein (IDP)Introduction of protein amyloid
授課老師：吳昆峯 |
|
第14週 |
5/28 |
Introduction of intrinsically disordered protein (IDP)
Introduction of protein amyloid (continued)
Molecular viewer and structural analysisIntroduction of molecular viewers: PyMOL
授課老師：吳昆峯 |
|
第15週 |
6/4 |
Practical course of PyMOL, view and analysis
Homework will be assigned.
授課老師：吳昆峯 |
|
第16週 |
6/11 |
Introduction of cryogenic electron microscopy (cryo-EM)Theory and practical aspectsApplicationData visualization and analysis.授課老師：吳昆峯 |
|
第17週 |
6/18 |
After course discussion
授課老師：陳佩燁 |
|
第18週 |
6/25 |
期末考 |